Brochure for the thermophiles 2015 meetingThe BFF has a long history in the production of thermophiles (see list at the bottom of this post).  Sterilize in place fermentors are well suited for production of these organisms since they require temperatures sometimes over 100 °C.  Autoclavable vessels can’t reach more than 65 °C in most cases.  Thermophiles have the added challenge of requiring anaerobic culture conditions.  Thermus thermophilus is commonly produced at the BFF and we were contracted by Venki Ramakrishnan for this strain which he used for purification of the ribosome.  Dr. Ramakrishnan was awarded the Nobel Prize in Chemistry for determining the structure of the ribosome.

rotor.pngThermus cells are easy to recognize as they have a chromophore that gives them an orange color as shown in the picture.  The cells grow at 70 °C and we typically culture these to mid-log phase which is optimal for ribosome expression.

The BFF is pleased to announce that Dr. Blum will be presenting at the Thermophiles 2015 conference in Santiago, Chile on September 4 (http://www.thermophiles2015.cl/).  This meeting will bring together experts in the area of thermophiles from across the globe with UGA’s Dr. Michael W.W. Adams and Juergen Weigel presenting along with Karl Stetter.

List of thermophiles BFF has experience with

  • Thermus thermophilus
  • Natranaerobius thermophilus
  • Pyrococcus furiosus
  • Methanocaldococcus jannaschii
  • Anaerocellum thermophilum
  • Sulfolobus solfataricus
  • Thermococcus litoralis
  • Thermotoga maritima
  • Bacillus halodenitricans
  • Thermoanaerobacter ethanolicus
  • Clostridium thermoautotrophicum
  • Clostridum thermocellum
  • Geobacillus stearothermophilus
  • Pyrobaculum aerophilum
  • Thermococcus barossii
  • Clostridium thermoaceticum
  • Thermus aquaticus
  • Thermus brockianus
  • Thermoanaerobacter X514
  • Thermococcus kodakaraensis
  • Thermoterrabacterium ferrireducens
  • Aquifex pyrophilus
  • Methanobacterium thermoautotrophicum
  • Desulfotomaculum nigri