The Bioexpression and Fermentation Facility is participating in a cancer reproducibility study in cooperation with Science Exchange and the Center for Open Science. Many labs have been involved and a report from NPR suggests that there are problems with reproducing work done in the original study. As described in the NPR report, previously, Amgen and Bayer had reported issues with reproducibility. The Cancer Reproducibility Study was developed to further evaluated those findings with the goal of "Through independent direct replication studies, the project aims to identify best practices that maximize reproducibility and facilitate an accurate accumulation of knowledge,... Read Full Article
The Bioexpression and Fermentation Facility is pleased to announce the addition of new capabilities for its training lab. TangenX recently donated a system capable of using 0.1 m2 membranes. This system will be used for training students in the use of tangential flow filtration which is widely used in bioprocessing. This system adds to the already wide array of equipment available in the training lab located in the Davison Life Sciences Complex on the campus of the University of Georgia. The lab, which is separate from BFF operations, is used for training of undergraduate and graduate students in protein expression and purification. Currently BCMB4030/6030 is taught to undergraduates in the Biochemistry department as well as students in... Read Full Article
Pittcon is the leading conference and exposition for laboratory science featuring new technology and scientific research. This year several folks from our team were able to attend the confernece. Here are some pictures from the outing.
Pictured (in order): Ruihua, Team Picture, Michelle, Tariq & Gabe
Services including immunizations, fusions and production of Hybridoma in cell culture. Five mice to be immunized and the highest titer mouse would be used for a fusion. The project includes immunizations, screening by ELISA, 1 fusion, screening of hybridomas and production of 2L of supernatant using the best clone selected.
Turnaround time: 6 months once the project initiated
Standard sample requirements: 2-4 mg of immunogen
Standard deliverables: purified antibody from 2L of culture
The Bioexpression and Fermentation Facility recently acquired the DasBox system from Eppendorf. The system is capable of independently controlling 4 x 250 mL bioreactors. This new capability will the BFF to accelerate process development projects in cell culture. Along with our Celligen 310 system we will be able to develop processes and scale to 40L within the facility. Since both systems are stirred tank reactors, scaling will be more predictable. The parallel processing capabilities will allow for Design of Experiments (DOE) methodologies.
What is Design of Experiments (DOE)?
Most people have faced this problem: I have a gene of interest (GOI) that I want to express, but how do I determine the best conditions for expression? The most common way is... Read Full Article
Recently I had the pleasure of presenting at the Euromabnet meeting held September 24-25 in Ljubljana, Slovenia. Slovenia, formerly part of Yugoslavia is nestled a short drive from Venice on the Adriatic sea and bordered by Croatia, Hungary, Italy and Austria.
The Euromabnet is a consortium of antibody researchers from across Europe including, Croatia, Czech Republic, France, Germany, Hungary, Italy, Netherlands, Russia, Slovia, Spain and Great Britain. According to their website, www.euromabnet.com, “EuroMAbNet represents the first European network of laboratories linked to academic Institutions, each having an internationally recognized reputation in the production and use of monoclonal antibodies. EuroMAbNet... Read Full Article
The BFF has a long history in the production of thermophiles (see list at the bottom of this post). Sterilize in place fermentors are well suited for production of these organisms since they require temperatures sometimes over 100 °C. Autoclavable vessels can’t reach more than 65 °C in most cases. Thermophiles have the added challenge of requiring anaerobic culture conditions. Thermus thermophilus is commonly produced at the BFF and we were contracted by Venki Ramakrishnan for this strain which he used for purification of the ribosome. Dr. Ramakrishnan was awarded the Nobel Prize in Chemistry for determining the structure of the ribosome.
BFF is proud to exhibit at the Society for Industrial Microbiology's Symposium for Biotechnology for Fuels and Chemicals to be held 28April - 30April 2015
This is a post-doctoral position within the Bioexpression and Fermentation Facility focusing on microbial fermentation. Qualified applicants can apply through the UGA Job Site (note - you may need to use your browser back button after clicking the link then click the link a second time to access the job posting)
This position has the following job duties:
- Conduct laboratory and pilot-scale experiments (up to 800L)
- Organize and maintain the fermentation development lab
- Identify, analyze and troubleshoot fermentation problems
- Develop, maintain and revise fermentation SOPs
The Bioexpression and Fermentation Facility (BFF) is accepting applications for a Research Professional level position in the protein purification group. The job responsibilities are as follows:
- Executing small and large-scale purification projects
- Running FPLC and HPLC chromatography systems for multi-mg or gram quantities of proteins
- Familiarity with TFF systems is also desirable as well as the ability to troubleshoot chromatography equipment issues as needed
- Write reports based on data collected from experiments according to facility guidelines and/or client specifications
- ... Read Full Article